ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 September 2002
Page Count: 5

Concordance Study on 15 STR Loci in Three Major Populations of Himalayan State Sikkim
Trivedi, R
Central Forensic Science Laboratorq, West Bengal

Guha, S
Central Forensic Science Laboratorq, West Bengal

Kashyap, VK
Central Forensic Science Laboratorq, West Bengal

Abstract

Samples were collected randomly from different individuals of East District of Sikkim. Nepali (N = 110). Bhutia ( N = 75) and Lepcha (N = 48) in form of either blood or buccal swab as per cooperation of participent. Genomic DNA was extracted by using standard phenol/chloroform procedure (2). Quantitation of DNA was carried out using the Quantiblot kit (PE Applied Biosystems) and subsequent PCR amplification was performed using the Powerplex^™ 16 multiplex System (Promega Coip, Madison, U.S.A.) The products were detected on a 5% denaturing polyacrylamide sequencing gel using the ABI Prism^™ 377 DNA Sequencer (PE Applied Biosystems) and genotype classification was made by comparison with allelic ladders provided with the Powerplex^™ 16 System.

Keywords:
forensic science DNA typing, STR, D3S1358, THO1, D21Sl1, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSFIPO, Penta D, YWA, D8S1179, TPOX, FGA, Sikkim population

Paper ID: JFS15547J
DOI: 10.1520/JFS15547J
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