Volume 47, Issue 5 (September 2002)
    Volume 47, Issue 5 (September 2002)

    Concordance Study on 15 STR Loci in Three Major Populations of Himalayan State Sikkim

    Published Online: September

    CODEN: JFSOAD

    Published Online: September

    CODEN: JFSOAD

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    Abstract

    Samples were collected randomly from different individuals of East District of Sikkim. Nepali (N = 110). Bhutia ( N = 75) and Lepcha (N = 48) in form of either blood or buccal swab as per cooperation of participent. Genomic DNA was extracted by using standard phenol/chloroform procedure (2). Quantitation of DNA was carried out using the Quantiblot kit (PE Applied Biosystems) and subsequent PCR amplification was performed using the Powerplex 16 multiplex System (Promega Coip, Madison, U.S.A.) The products were detected on a 5% denaturing polyacrylamide sequencing gel using the ABI Prism 377 DNA Sequencer (PE Applied Biosystems) and genotype classification was made by comparison with allelic ladders provided with the Powerplex 16 System.


    Author Information:

    Trivedi, R
    Central Forensic Science Laboratorq, Kolkata, West Bengal

    Guha, S
    Central Forensic Science Laboratorq, Kolkata, West Bengal

    Kashyap, VK
    Central Forensic Science Laboratorq, Kolkata, West Bengal


    Stock #: JFS15547J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15547J

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    Author
    Title Concordance Study on 15 STR Loci in Three Major Populations of Himalayan State Sikkim
    Symposium , 0000-00-00
    Committee E30