Volume 47, Issue 5 (September 2002)
Genotype Profile for Thirteen Tetranucleotide Repeat Loci and Two Pentanucleotide Repeat Loci in Four Endogamous Tamil Population Groups of India
Blood samples were obtained by venipuncture from random individuals of the populations: Tanjore Kallar (101), Vanniyar (87), Pallar (33) and Paraiyar (21). DNA was isolated using standard phenol/chloroform procedure (1). The 13 tetranucleotide repeat loci and two pentanucleotide repeat loci were amplified using the PowerPlex® 16 system (Promega Corporation, Madison) according to manufacturer's recommendations (2). The amplified products were separated and detected using the ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems, Foster City). The data were analyzed using DNATYPE program (3). The genotype frequencies of the 15 loci studied in the four endogamous Tamil populations are given in Tables 1–4. Statistical results for G-square test, exact test, observed and expected homozygosity are presented in Tables 5–8. The data are consistent with that generated on other studied Indian population groups (4–7).