Volume 47, Issue 5 (September 2002)
Genetic Variation at Five STR Loci (D10S2325, D13S325, D20S470, D18S51, and D19S253) in Korean Population
Allele and genotype frequencies of the five STR loci (D10S2325, D13S325, D20S470, D18S51, and D19S253) were determined. Specimens were collected from unrelated volunteer blood donors from a population of Chungnam Province in South Korea (n = 130). DNA was obtained from the blood specimens using the Wizard genomic DNA purification kit (Promega, USA) and amplified using the multiplex PCR (triplex amplification of D10S2325, D13S325, and D20S470; duplex amplification of D18S51 and D19S253). The amplified DNAs were separated by denaturing polyacrylamide gel electrophoresis and then visualized by the silver staining (1). Exact x2-test for Hardy-Weinberg equilibrium and pairwise disequilibrium were analyzed using the GDA program written by Lewis P.O. and Zaykin D (2). The chance of exclusion of combined system was 0.9986.