Volume 47, Issue 5 (September 2002)

    The Use of SEM/EDS Analysis to Distinguish Dental and Osseus Tissue from Other Materials

    (Received 29 April 2002; accepted 7 April 2002)

    Published Online: September


      Format Pages Price  
    PDF 4 $25   ADD TO CART


    With increasing frequency, relatively small, fragmentary evidence thought to be osseous or dental tissue of human origin is submitted to the forensic laboratory for DNA analysis with the request for positive identification. Prior to performing DNA analysis, however, it is prudent to first perform a presumptive test or “screen” to determine whether the questioned material may be eliminated from further consideration. When material is shown not to be consistent with bone/teeth, DNA testing is not performed. When such determinations cannot be made from gross morphological features, elemental analysis can be indicative.

    This presumptive test is made possible by applying scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS) in conjunction with an X-ray spectral database recently developed by the FBI laboratory. This database includes spectra for many different materials including known examples of bone and tooth from many different contexts and representing the full range of taphonomic conditions. Results of SEM/EDS analysis of evidence can be compared to these standards to determine if they are consistent with bone and/or tooth and, if not, then what the material might represent. Analysis suggests that although the proportions and amounts of calcium and phosphorus are particularly important in differentiating bone and tooth from other materials, other minor differences in spectral profile can also provide significant discrimination. Analysis enables bone and tooth to be successfully distinguished from other materials in most cases. Exceptions appear to be ivory, mineral apatite, and perhaps some types of corals.

    Author Information:

    Ward, DC
    Federal Bureau of Investigation, Washington, DC,

    Ubelaker, DH
    National Museum of Natural History Smith-sonian Institution, Washington, DC,

    Stewart, J
    Federal Bureau of Investigation, Washington, DC,

    Braz, VS
    Federal University of Rio de Janeiro,

    Stock #: JFS15518J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15518J

    ASTM International
    is a member of CrossRef.

    Title The Use of SEM/EDS Analysis to Distinguish Dental and Osseus Tissue from Other Materials
    Symposium , 0000-00-00
    Committee E30