Successful DNA Typing of a Urine Sample in a Doping Control Case Using Human Mitochondrial DNA Analysis

    Volume 47, Issue 5 (September 2002)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 September 2002

    Page Count: 3


    Junge, A
    Institute of Legal Medicine Rheinische Friedrich-Wilhelms-University Bonn, Bonn,

    Madea, B
    Institute of Legal Medicine Rheinische Friedrich-Wilhelms-University Bonn, Bonn,

    Steevens, M
    Institute of Legal Medicine Rheinische Friedrich-Wilhelms-University Bonn, Bonn,

    (Received 8 April 2002; accepted 23 March 2002)

    Abstract

    In a doping control case, a urine sample was tested positive for nandrolon. We were asked by the athlete to perform DNA investigations on the questioned urine sample and compare these to a fresh blood sample taken from the athlete in order to detect or rule out manipulation and/or switching of the samples. The urine sample had been collected nine months prior to the investigation and had been stored at 4°C. In a first approach, nuclear DNA systems were investigated that failed with the exception of the Amelogenin system. Due to the high copy number of mitochondrial DNA molecules and the robustness of the mitochondrial genome, we investigated the HVR I and HVR II regions of mitochondrial DNA and obtained reproducible and clear sequencing results for both the blood and the urine samples. Due to the identical sequences, it could not be excluded that the blood sample and the urine sample were from the same individual or an individual having the same maternal lineage.


    Paper ID: JFS15509J

    DOI: 10.1520/JFS15509J

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    Title Successful DNA Typing of a Urine Sample in a Doping Control Case Using Human Mitochondrial DNA Analysis
    Symposium , 0000-00-00
    Committee E30