ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 September 2002
Page Count: 3

Extraction of Human Nuclear DNA from Feces Samples Using the Qiaamp DNA Stool Mini Kit
Vandenberg, N
Victoria Forensic Science Centre, Victoria Police, Macleod,

Van Oorschot, RAH
Victoria Forensic Science Centre, Victoria Police, Macleod,

(Received 5 March 2002; accepted 18 February 2001)

Abstract

The use of a QIAamp DNA Stool Mini Kit (QIAGEN) for extracting human nuclear DNA from feces samples is reported. This method employs a stool lysis buffer and a unique matrix (InhibitEX tablet) to remove PCR inhibitory substances specific to feces samples. DNA extracted from various amounts of stool and from stool samples exposed to different environmental impacts was successfully amplified and typed using the Profiler Plus Amplification Kit and ABI PRISM 310 Genetic Analyser.

Keywords:
forensic science, DNA extraction, feces sample, genotyping, polymerase chain reaction inhibitors, environmental study

Paper ID: JFS15502J
DOI: 10.1520/JFS15502J
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