Journal Published Online: 01 September 2002
Volume 47, Issue 5

Extraction of Human Nuclear DNA from Feces Samples Using the Qiaamp DNA Stool Mini Kit

CODEN: JFSCAS

Abstract

The use of a QIAamp DNA Stool Mini Kit (QIAGEN) for extracting human nuclear DNA from feces samples is reported. This method employs a stool lysis buffer and a unique matrix (InhibitEX tablet) to remove PCR inhibitory substances specific to feces samples. DNA extracted from various amounts of stool and from stool samples exposed to different environmental impacts was successfully amplified and typed using the Profiler Plus Amplification Kit and ABI PRISM 310 Genetic Analyser.

Author Information

Vandenberg, N
Victoria Forensic Science Centre, Victoria Police, Macleod, Victoria, Australia
Van Oorschot, RAH
Victoria Forensic Science Centre, Victoria Police, Macleod, Victoria, Australia
Pages: 3
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Stock #: JFS15502J
ISSN: 0022-1198
DOI: 10.1520/JFS15502J