Volume 47, Issue 5 (September 2002)

    Extraction of Human Nuclear DNA from Feces Samples Using the Qiaamp DNA Stool Mini Kit

    (Received 5 March 2002; accepted 18 February 2001)

    Published Online: September

    CODEN: JFSOAD

      Format Pages Price  
    PDF 3 $25   ADD TO CART


    Abstract

    The use of a QIAamp DNA Stool Mini Kit (QIAGEN) for extracting human nuclear DNA from feces samples is reported. This method employs a stool lysis buffer and a unique matrix (InhibitEX tablet) to remove PCR inhibitory substances specific to feces samples. DNA extracted from various amounts of stool and from stool samples exposed to different environmental impacts was successfully amplified and typed using the Profiler Plus Amplification Kit and ABI PRISM 310 Genetic Analyser.


    Author Information:

    Vandenberg, N
    Victoria Forensic Science Centre, Victoria Police, Macleod, Victoria,

    Van Oorschot, RAH
    Victoria Forensic Science Centre, Victoria Police, Macleod, Victoria,


    Stock #: JFS15502J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15502J

    ASTM International
    is a member of CrossRef.

    Author
    Title Extraction of Human Nuclear DNA from Feces Samples Using the Qiaamp DNA Stool Mini Kit
    Symposium , 0000-00-00
    Committee E30