Extraction of Human Nuclear DNA from Feces Samples Using the Qiaamp DNA Stool Mini Kit

    Volume 47, Issue 5 (September 2002)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 September 2002

    Page Count: 3


    Vandenberg, N
    Victoria Forensic Science Centre, Victoria Police, Macleod, Victoria,

    Van Oorschot, RAH
    Victoria Forensic Science Centre, Victoria Police, Macleod, Victoria,

    (Received 5 March 2002; accepted 18 February 2001)

    Abstract

    The use of a QIAamp DNA Stool Mini Kit (QIAGEN) for extracting human nuclear DNA from feces samples is reported. This method employs a stool lysis buffer and a unique matrix (InhibitEX tablet) to remove PCR inhibitory substances specific to feces samples. DNA extracted from various amounts of stool and from stool samples exposed to different environmental impacts was successfully amplified and typed using the Profiler Plus Amplification Kit and ABI PRISM 310 Genetic Analyser.


    Paper ID: JFS15502J

    DOI: 10.1520/JFS15502J

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    Author
    Title Extraction of Human Nuclear DNA from Feces Samples Using the Qiaamp DNA Stool Mini Kit
    Symposium , 0000-00-00
    Committee E30