Journal Published Online: 01 September 2002
Volume 47, Issue 5

Skeletal Remains Presumed Submerged in Water for Three Years Identified Using PCR-STR Analysis

CODEN: JFSCAS

Abstract

We describe the successful identification of the remains of a saponified body found in a dam by typing of nuclear DNA. Whereas DNA extracted from soft tissues yielded negative PCR results, DNA extracted from the bone by a slightly modified Qiagen procedure allowed the typing of sex (AMG locus) and of 10 additional STR loci. An identity document was found belonging to a man missing for 3 years and comparison of the results to the DNA profiles of his son and wife confirmed the identity. The longest delay reported until now for successful nuclear DNA geno-typing after immersion in river water was 18 months. This case demonstrates a delay of up to 3 years.

Author Information

Crainic, K
Laboratoire de Biochimie et Biologie Moléculaire, Hôpital R. Poincaré, Garches Faculté de Médecine Paris-Ouest, Garches Institut Universitaire de Médecine Légale et Droit Médical, Paris
Paraire, F
Service de Médecine Légale et Anatomie Pathologique, Hôpital R. Poincaré, Garches
Leterreux, M
Laboratoire de Biochimie et Biologie Moléculaire, Hôpital R. Poincaré, Garches
Durigon, M
Faculté de Médecine Paris-Ouest, Garches Service de Médecine Légale et Anatomie Pathologique, Hôpital R. Poincaré, Garches
de Mazancourt, P
Laboratoire de Biochimie et Biologie Moléculaire, Hôpital R. Poincaré, Garches Laboratoire de Biochimie et Biologie Moléculaire, Hôpital R. Poincaré, Garches
Pages: 3
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Stock #: JFS15490J
ISSN: 0022-1198
DOI: 10.1520/JFS15490J