Volume 47, Issue 5 (September 2002)

    Skeletal Remains Presumed Submerged in Water for Three Years Identified Using PCR-STR Analysis

    (Received 8 April 2002; accepted 23 March 2002)

    Published Online: September

    CODEN: JFSOAD

      Format Pages Price  
    PDF 3 $25   ADD TO CART


    Abstract

    We describe the successful identification of the remains of a saponified body found in a dam by typing of nuclear DNA. Whereas DNA extracted from soft tissues yielded negative PCR results, DNA extracted from the bone by a slightly modified Qiagen procedure allowed the typing of sex (AMG locus) and of 10 additional STR loci. An identity document was found belonging to a man missing for 3 years and comparison of the results to the DNA profiles of his son and wife confirmed the identity. The longest delay reported until now for successful nuclear DNA geno-typing after immersion in river water was 18 months. This case demonstrates a delay of up to 3 years.


    Author Information:

    Paraire, F
    Service de Médecine Légale et Anatomie Pathologique, Hôpital R. Poincaré, Garches,

    Crainic, K

    Durigon, M

    Leterreux, M
    Laboratoire de Biochimie et Biologie Moléculaire, Hôpital R. Poincaré, Garches,

    de Mazancourt, P


    Stock #: JFS15490J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15490J

    ASTM International
    is a member of CrossRef.

    Author
    Title Skeletal Remains Presumed Submerged in Water for Three Years Identified Using PCR-STR Analysis
    Symposium , 0000-00-00
    Committee E30