Genetic Variation at Three DYS-STR Loci and Amelogenin Between the Australian Caucasians and Three Asian Ethnic Groups

    Volume 47, Issue 4 (July 2002)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 July 2002

    Page Count: 4


    Both, K
    Forensic Science Centre, Adelaide, SA

    Burgoyne, LA
    School of Biological Sciences, Flinders University of South Australia, Bedford Park, SA

    Chang, YM
    Forensic Science Centre, Adelaide, SA

    Abstract

    Blood samples were obtained from unrelated male individuals from the four population groups and placed on FTA® paper (Fitzco-Whatman). DNA was extracted using the aqueous processing method for FTA® paper. One extracted disc (1.2 mm diameter) was used in the PCR per sample. The mixed male DNA sample was loaded onto a pre-washed FTA® paper and subjected to an alkalineisopropanol treatment. A combination of three Y-linked microsatellites loci, DYS438 (1), DYS390 (2), and DYS439 (1) and amelogenin were amplified simultaneously using a fluorescent multiplex system. DYS438 and amelogenin were labelled with 6-FAM, DYS390 with JOE and DYS439 with TAMRA.


    Paper ID: JFS15483J

    DOI: 10.1520/JFS15483J

    ASTM International
    is a member of CrossRef.

    Author
    Title Genetic Variation at Three DYS-STR Loci and Amelogenin Between the Australian Caucasians and Three Asian Ethnic Groups
    Symposium , 0000-00-00
    Committee E30