ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 July 2002
Page Count: 4

Genetic Variation at Three DYS-STR Loci and Amelogenin Between the Australian Caucasians and Three Asian Ethnic Groups
Both, K
Forensic Science Centre, SA

Burgoyne, LA
School of Biological Sciences, Flinders University of South Australia, SA

Chang, YM
Forensic Science Centre, SA

Abstract

Blood samples were obtained from unrelated male individuals from the four population groups and placed on FTA® paper (Fitzco-Whatman). DNA was extracted using the aqueous processing method for FTA® paper. One extracted disc (1.2 mm diameter) was used in the PCR per sample. The mixed male DNA sample was loaded onto a pre-washed FTA® paper and subjected to an alkalineisopropanol treatment. A combination of three Y-linked microsatellites loci, DYS438 (1), DYS390 (2), and DYS439 (1) and amelogenin were amplified simultaneously using a fluorescent multiplex system. DYS438 and amelogenin were labelled with 6-FAM, DYS390 with JOE and DYS439 with TAMRA.

Keywords:
forensic science, DNA typing, population genetics, DYS438, DYS390, DYS439, Australia, Malaysia

Paper ID: JFS15483J
DOI: 10.1520/JFS15483J
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