ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 July 2002
Page Count: 5

Allele Frequency Data for Powerplex 16 Loci in Four Major Populations of Orissa, India
Sahoo, S
DNA Typing Unit, Central Forensic Science Laboratory,

Kashyap, VK
DNA Typing Unit, Central Forensic Science Laboratory,

Abstract

Genomic DNA was obtained from blood samples by using standard phenol/chloroform procedure (2). Quantitation of DNA was carried out using the Quantiblot kit (PE Applied Biosystems) and subsequent PCR amplification was performed using the Powerplex ™ 16 System (Promega Corp.) The products were detected on a 5% denaturing polyacrylamide sequencing gels using the ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems) and genotype classification was made by comparison with allelic ladders provided with the Powerplex™ 16 System.

Keywords:
forensic science, population genetics, DNA typing, polymorphism, short tandem repeat, D3S1358, THO1, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, Oriya population

Paper ID: JFS15482J
DOI: 10.1520/JFS15482J
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