Volume 47, Issue 4 (July 2002)

    Allele Frequency Data for Powerplex 16 Loci in Four Major Populations of Orissa, India

    Published Online: July

    CODEN: JFSOAD

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    Abstract

    Genomic DNA was obtained from blood samples by using standard phenol/chloroform procedure (2). Quantitation of DNA was carried out using the Quantiblot kit (PE Applied Biosystems) and subsequent PCR amplification was performed using the Powerplex ™ 16 System (Promega Corp.) The products were detected on a 5% denaturing polyacrylamide sequencing gels using the ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems) and genotype classification was made by comparison with allelic ladders provided with the Powerplex™ 16 System.


    Author Information:

    Sahoo, S
    DNA Typing Unit, Central Forensic Science Laboratory, West Bengal,

    Kashyap, VK
    DNA Typing Unit, Central Forensic Science Laboratory, West Bengal,


    Stock #: JFS15482J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15482J

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    Author
    Title Allele Frequency Data for Powerplex 16 Loci in Four Major Populations of Orissa, India
    Symposium , 0000-00-00
    Committee E30