HLA-DQA1 and Polymarker Loci Allele Frequencies in the Bharias, a Primitive Indian Tribal Population from Patalkot, India

    Volume 47, Issue 4 (July 2002)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 July 2002

    Page Count: 2


    Biswas, R
    Central Forensic Science Laboratory, Chandigarh,

    Giroti, R
    Central Forensic Science Laboratory, Chandigarh,

    Abstract

    Twenty-three specimens were collected from unrelated volunteer blood donors. DNA was obtained from blood specimens using “QIAamp Blood and Tissue kit” (QIAGEN GmbH, Hilden, Germany) according to the manufacturer's recommended protocol. Amplification was carried out in a “Gene Amp® PCR system 2400” thermal cycler (Perkin Elmer Corporation, California) using 5 ng target DNA. Typing of the six loci was performed by reverse dot blot with Allele Specific Oligonucleotides (ASO) probes, using “AmpliType® PMHLADQA1 PCR amplification and typing kit” (Roche Molecular Systems, Inc., New Jersey).


    Paper ID: JFS15481J

    DOI: 10.1520/JFS15481J

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    Title HLA-DQA1 and Polymarker Loci Allele Frequencies in the Bharias, a Primitive Indian Tribal Population from Patalkot, India
    Symposium , 0000-00-00
    Committee E30