Volume 47, Issue 4 (July 2002)
Allele Frequencies of HLADQA1 and PM in Istanbul (Turkey) Population
Blood samples were obtained from randomly selected, healthy, unrelated individuals from both sexes and were examined in order to estimate the allele and genotype frequencies of HLADQA1, LDLR, GYPA, HBGG, D7S8, GC loci. DNA was extracted using Chelex 100 extraction method (1). The amplification was carried out according to the instructions of Amplitype PMDQA1 PCR amplification kit. The samples were typed for HLADQA1/PM loci using the Amplitype PM + DQA1 kit (Perkin Elmer, Foster City, CA) as per the manufacturer's instructions. The statistical analysis was done by the gene count method and it revealed no significant deviation from Hardy-Weinberg expectations. The complete data including the comparison with various Turkish and different populations (2) and the statistical parameters of medico/legal interest are available at http://istanbul.edu.tr/enstituler/forensic/popgen-04.htm.