Volume 41, Issue 2 (March 1996)

    ABO Genotyping Following a Single PCR Amplification

    (Received 27 February 1995; accepted 16 August 1995)

    CODEN: JFSOAD

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    Abstract

    Using primers designed by Lee and Chang, 200 base-pair (bp) fragment of ABO locus was amplified by PCR, which spans the site of the single nucleotide deletion associated with O allele. O allele could be identified by Kpn I digestion of the PCR product as reported. A and B alleles were also distinguishable by Mae II digestion of the product. Thus restriction digestion by Kpn I and Mae II could genotype ABO blood group following the single amplification. The nucleotide substitution in the 200-bp product between A and B alleles was also found in O allele, resulting in 2 different suballeles OA and OG. The single-strand conformational polymorphism of the PCR product was also investigated for ABO genotyping following the single amplification.


    Author Information:

    Akane, A
    Professor and Forensic Scientists, Kansai Medical University, Moriguchi, Osaka

    Kimura, K
    Associate Professor and Professor, Shimane Medical University, Izumo,

    Matsubara, K
    Associate Professor and Professor, Shimane Medical University, Izumo,

    Yoshida, M
    Professor and Forensic Scientists, Kansai Medical University, Moriguchi, Osaka

    Yoshimura, S
    Professor and Forensic Scientists, Kansai Medical University, Moriguchi, Osaka

    Watabiki, T
    Professor and Forensic Scientists, Kansai Medical University, Moriguchi, Osaka

    Okii, Y
    Professor and Forensic Scientists, Kansai Medical University, Moriguchi, Osaka


    Stock #: JFS15425J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15425J

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    Author
    Title ABO Genotyping Following a Single PCR Amplification
    Symposium , 0000-00-00
    Committee E30