Journal Published Online: 01 March 1996
Volume 41, Issue 2

ABO Genotyping Following a Single PCR Amplification

CODEN: JFSCAS

Abstract

Using primers designed by Lee and Chang, 200 base-pair (bp) fragment of ABO locus was amplified by PCR, which spans the site of the single nucleotide deletion associated with O allele. O allele could be identified by Kpn I digestion of the PCR product as reported. A and B alleles were also distinguishable by Mae II digestion of the product. Thus restriction digestion by Kpn I and Mae II could genotype ABO blood group following the single amplification. The nucleotide substitution in the 200-bp product between A and B alleles was also found in O allele, resulting in 2 different suballeles OA and OG. The single-strand conformational polymorphism of the PCR product was also investigated for ABO genotyping following the single amplification.

Author Information

Akane, A
Kansai Medical University, Moriguchi, Osaka, Japan
Yoshimura, S
Kansai Medical University, Moriguchi, Osaka, Japan
Yoshida, M
Kansai Medical University, Moriguchi, Osaka, Japan
Okii, Y
Kansai Medical University, Moriguchi, Osaka, Japan
Watabiki, T
Kansai Medical University, Moriguchi, Osaka, Japan
Matsubara, K
Shimane Medical University, Izumo, Japan
Kimura, K
Shimane Medical University, Izumo, Japan
Pages: 3
Price: $25.00
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Stock #: JFS15425J
ISSN: 0022-1198
DOI: 10.1520/JFS15425J