ISSN: 0022-1198
CODEN: JFSCA
Page Count: 3

ABO Genotyping Following a Single PCR Amplification
Akane, A
Professor and Forensic Scientists, Kansai Medical University, Osaka

Kimura, K
Associate Professor and Professor, Shimane Medical University,

Matsubara, K
Associate Professor and Professor, Shimane Medical University,

Yoshida, M
Professor and Forensic Scientists, Kansai Medical University, Osaka

Yoshimura, S
Professor and Forensic Scientists, Kansai Medical University, Osaka

Watabiki, T
Professor and Forensic Scientists, Kansai Medical University, Osaka

Okii, Y
Professor and Forensic Scientists, Kansai Medical University, Osaka

(Received 27 February 1995; accepted 16 August 1995)

Abstract

Using primers designed by Lee and Chang, 200 base-pair (bp) fragment of ABO locus was amplified by PCR, which spans the site of the single nucleotide deletion associated with O allele. O allele could be identified by Kpn I digestion of the PCR product as reported. A and B alleles were also distinguishable by Mae II digestion of the product. Thus restriction digestion by Kpn I and Mae II could genotype ABO blood group following the single amplification. The nucleotide substitution in the 200-bp product between A and B alleles was also found in O allele, resulting in 2 different suballeles O^A and O^G. The single-strand conformational polymorphism of the PCR product was also investigated for ABO genotyping following the single amplification.

Keywords:
forensic science, DNA, ABO blood group system, PCR, restriction endonuclease, single-strand conformational polymorphism (SSCP)

Paper ID: JFS15425J
DOI: 10.1520/JFS15425J
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