Journal Published Online: 01 March 1995
Volume 40, Issue 2

Consistency and Reproducibility of AmpliType® PM Results Between Seven Laboratories: Field Trial Results

CODEN: JFSCAS

Abstract

The AmpliType® PM Field Trial was designed to assess the ability of forensic laboratories to obtain the correct results from samples commonly encountered in forensic casework. The seven forensic laboratory participants of the AmpliType® PM Field Trial each performed four studies. Samples were analyzed using components of the AmpliType® PM PCR Amplification and Typing Kit. Laboratories were also provided with DNA probe strips to type the DQA1 locus. Of the 381 PM and 325 DQA1 DNA probe strip results obtained from DNA-containing and non-DNA-containing samples, 98.2% and 95.7% showed the correct result for PM and DQA1 types, respectively. No samples were typed incorrectly. The remaining small percentage of samples were either uninterpretable due to the presence of a mixture, or no result was obtained due to insufficient DNA. The Field Trial demonstrated that laboratories can easily implement the AmpliType® PM system to analyze DNA-containing samples and controls successfully for forensic casework applications.

Author Information

Fildes, N
Department of Human Genetics, Roche Molecular Systems, Inc., Alameda, CA
Reynolds, R
Department of Human Genetics, Roche Molecular Systems, Inc., Alameda, CA
Pages: 8
Price: $25.00
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Stock #: JFS15358J
ISSN: 0022-1198
DOI: 10.1520/JFS15358J