ISSN: 0022-1198
CODEN: JFSCA
Page Count: 4

AmpliType® PM and HLA DQα Typing from Pap Smear, Semen Smear, and Postcoital Slides
Reynolds, R
Human Identity Section Manager, Research and Development, Roche Molecular Systems, Inc., CA

Roy, R
Criminalist Supervisor, N.S.P. Criminalistics Laboratory, NE

(Received 22 February 1994; accepted 5 August 1994)

Abstract

Deoxyribonucleic acid (DNA) samples extracted from stained pap smear, semen smear and postcoital slides were amplified by the polymerase chain reaction (PCR) and typed for the AmpliType® PM and HLA DQα alleles. HLA DQ α and PM types consistent with blood controls from the donors were obtained. Stained cells fixed to slides can provide a valuable source of material for determining a genetic profile of the sample donor, particularly in sexual assault cases and possibly in missing person cases involving women.

Keywords:
criminalistics, DNA, AmpliType® PM, HLA DQα, PCR, sexual assault evidence

Paper ID: JFS15355J
DOI: 10.1520/JFS15355J
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