Development of a Fluoroimmunoassay for the Detection of Buprenorphine in Urine

    Volume 40, Issue 2 (March 1995)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 4


    Debrabandere, L
    Research Associate, Senior Research Associate, and Professor of Toxicology, University of Leuven, Laboratory of Toxicology, Leuven,

    Van Boven, M
    Research Associate, Senior Research Associate, and Professor of Toxicology, University of Leuven, Laboratory of Toxicology, Leuven,

    Daenens, P
    Research Associate, Senior Research Associate, and Professor of Toxicology, University of Leuven, Laboratory of Toxicology, Leuven,

    (Received 26 July 1993; accepted 2 August 1994)

    Abstract

    The development of a fluoroimmunoassay for the detection of buprenorphine in urine samples is described. Fluore-scein-norbuprenorphine and pseudobuprenorphine, the dimer of buprenorphine, were synthesized as tracer molecules. The antibodies were prepared by coupling the 2-diazobenzoic acid derivative of buprenorphine with bovine serum albumine, using the carbodiimide method. The assay was mainly used for the routine detection of buprenorphine in urine specimens of persons suspected of Temgesic® abuse. The minimum detectable dose of the immunoassay was calculated to be 20 ng/mL.


    Paper ID: JFS15352J

    DOI: 10.1520/JFS15352J

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    Title Development of a Fluoroimmunoassay for the Detection of Buprenorphine in Urine
    Symposium , 0000-00-00
    Committee E30