Journal Published Online: 01 March 1995
Volume 40, Issue 2

Development of a Fluoroimmunoassay for the Detection of Buprenorphine in Urine

CODEN: JFSCAS

Abstract

The development of a fluoroimmunoassay for the detection of buprenorphine in urine samples is described. Fluore-scein-norbuprenorphine and pseudobuprenorphine, the dimer of buprenorphine, were synthesized as tracer molecules. The antibodies were prepared by coupling the 2-diazobenzoic acid derivative of buprenorphine with bovine serum albumine, using the carbodiimide method. The assay was mainly used for the routine detection of buprenorphine in urine specimens of persons suspected of Temgesic® abuse. The minimum detectable dose of the immunoassay was calculated to be 20 ng/mL.

Author Information

Debrabandere, L
University of Leuven, Laboratory of Toxicology, Leuven, Belgium
Van Boven, M
University of Leuven, Laboratory of Toxicology, Leuven, Belgium
Daenens, P
University of Leuven, Laboratory of Toxicology, Leuven, Belgium
Pages: 4
Price: $25.00
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Stock #: JFS15352J
ISSN: 0022-1198
DOI: 10.1520/JFS15352J