Volume 47, Issue 3 (May 2002)
Frequency Data of Nine Y-Chromosomal STR Loci in a Sample from Central Spain
A sample of 91 blood specimens from unrelated apparently healthy males was collected from individuals autochthonous to the geographic region of Vera-Jerte Valleys, Central Spain (the four grandparents were born within the region). DNA was recovered using a standard phenol/chloroform procedure and typed in an ALF-Sequencer (Pharmacia) according to protocols and alleles kindly supplied by Peter de Knijff (1) for DYS390, DYS391, DYS393, DYS19 loci. DYS434, DYS437, and DYS439 systems were typed according to Hou et al. (2) and DYS389I and DYS389II were typed according to Schultes et al. (3) using allelic ladders from our laboratory. Frequencies were calculated through the gene counting method and gene diversity was estimated according to Nei (4).