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DNA samples from 101 unrelated individuals were extracted by Chelex procedure (1) and then quantified using QuantiBlot® Human DNA Quantitation Kit according to the manufacturer's instructions (2). DNA samples (1 ng) were amplified and typed by PowerPlex® 16 System (3). The electrophoresis was carried out on the ABI PRISM® 377 DNA Sequencer using GeneScan® Genotyper® and PowerTyper™ 16 Macro software. Data were analyzed using a program provided by R. Chakraborty (University of Texas School of Biomedical Sciences, Houston, Texas). Interclass correlations yielding p < 0.05 for pairwise comparisons: TH01/D7S820, TH01/D8S1179, D18S51/D5S818, D18S51/D7S820, PentaE/PentaD, and D5S818/D16S539.
Forensic Science, FBI Academy, VA
University of Granada,
Stock #: JFS15276J