Volume 47, Issue 2 (March 2002)

    Allele Frequencies for the AmpFlSTR Profiler Loci in a Colombian Population (Department of Boyaca)

    Published Online: March

    CODEN: JFSOAD

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    Abstract

    Whole blood samples were obtained in the genetics laboratory of the UIS (Universidad Industrial of Santander) and Boyaca Regional ICBF (Instituto Colombiano de Bienestar Familiar) from 183 unrelated Colombian donors (Department of Boyaca). Genomic DNA was extracted using the salting out procedure. PCR amplification was performed using the AmpFlSTR Profiler amplification kit (PE-Biosystems, Foster City, CA) following manufacturer's instructions. The amplified products were separated and detected using the ABI 310 DNA sequencer (PE-Biosystems, Foster City, CA). Alleles were classified according to the recommendations of the ISFH (1). Statistical analysis was performed as previously reported (2). The complete data set is available to any interested researcher upon request from the corresponding author, Oscar Garcia, Ph.D.


    Author Information:

    Pico, AL
    Laboratorio de Genetica, Facultad de Salud, Universidad Industrial de Santander (UIS), Bucaramanga,

    Gil, AM
    Laboratorio de Genetica, Facultad de Salud, Universidad Industrial de Santander (UIS), Bucaramanga,

    Castillo, A
    Laboratorio de Genetica, Facultad de Salud, Universidad Industrial de Santander (UIS), Bucaramanga,

    Vargas, CI
    Laboratorio de Genetica, Facultad de Salud, Universidad Industrial de Santander (UIS), Bucaramanga,

    Garcia, O
    Area de Laboratorio Ertzaintza, Bilbao,


    Stock #: JFS15270J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15270J

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    Author
    Title Allele Frequencies for the AmpFlSTR Profiler Loci in a Colombian Population (Department of Boyaca)
    Symposium , 0000-00-00
    Committee E30