Forensic Comparison of Soils by Bacterial Community DNA Profiling

    Volume 47, Issue 2 (March 2002)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 March 2002

    Page Count: 4


    Osborn, AM
    University of Essex, Wivenhoe Park, Colchester

    Nogales, B
    University of Essex, Wivenhoe Park, Colchester

    Maas, EW
    The Institute of Environmental Science and Research Limited (ESR), Kenepuru Science Centre, Porirua,

    Horswell, J
    The Institute of Environmental Science and Research Limited (ESR), Kenepuru Science Centre, Porirua,

    Sutherland, KBW
    The Institute of Environmental Science and Research Limited (ESR), Kenepuru Science Centre, Porirua,

    Cordiner, SJ
    The Institute of Environmental Science and Research Limited (ESR), Kenepuru Science Centre, Porirua,

    Martin, TM
    The Institute of Environmental Science and Research Limited (ESR), Kenepuru Science Centre, Porirua,

    Speir, TW
    The Institute of Environmental Science and Research Limited (ESR), Kenepuru Science Centre, Porirua,

    (Received 20 August 2001; accepted 13 August 2001)

    Abstract

    This preliminary investigation has shown that a soil microbial community DNA profile can be obtained from the small sample of soil recovered from the sole of a shoe, and from soil stains on clothing. We have also shown that these profiles are representative of the site of collection and therefore could potentially be used as associative evidence to prove a link between suspects and crime scenes. Soil community profiles were obtained using the T-RFLP fingerprinting method that uses fluorescent primer technology and semi-automated analysis techniques similar to those used in human DNA profiling in forensic laboratories.


    Paper ID: JFS15256J

    DOI: 10.1520/JFS15256J

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    Title Forensic Comparison of Soils by Bacterial Community DNA Profiling
    Symposium , 0000-00-00
    Committee E30