Determination of ABO Blood Grouping from Human Oral Squamous Epithelium by the Highly Sensitive Immunohistochemical Staining Method EnVision+

    Volume 47, Issue 2 (March 2002)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 March 2002

    Page Count: 4


    Noda, H
    Kinki University School of Medicine, Osaka,

    Yokota, M
    Kinki University School of Medicine, Osaka,

    Sugiyama, S
    Kinki University School of Medicine, Osaka,

    Tatsumi, S
    Kinki University School of Medicine, Osaka,

    (Received 23 June 2001; accepted 18 June 2001)

    Abstract

    Using the highly sensitive immunohistochemical staining method EnVision+, which employs a dextran polymer reagent for the secondary antibody, the detection of the ABH antigens was attempted in the oral squamous epithelium. This new technique uses monoclonal antibody as a primary antibody and it takes about three hours for staining. The time is much shorter than conventional absorption-elution testing or absorption-inhibition testing for the determination of ABO blood grouping. Secretor saliva samples were stained at strong intensity by the antibody, which corresponded to its blood group and anti-H. On the one hand, nonsecretor saliva samples were stained at strong intensity only by the antibody that corresponded to its blood group, and at weak intensity only by anti-H. Since human oral squamous epithelium antigens were stained specifically by this method, we can examine the ABO blood group of saliva samples and perform cytodiagnosis at the same time.

    Our research suggested that the EnVision+ Method is a useful technique for ABO blood grouping of saliva in forensic cases.


    Paper ID: JFS15254J

    DOI: 10.1520/JFS15254J

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    Title Determination of ABO Blood Grouping from Human Oral Squamous Epithelium by the Highly Sensitive Immunohistochemical Staining Method EnVision+
    Symposium , 0000-00-00
    Committee E30