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    Volume 47, Issue 1 (January 2002)

    Haplotype Frequencies for Two New Y-STR Loci in Chinese Population

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    Blood samples were collected from 104 unrelated male individuals of Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). Primers for Y-GATA-C4 were redesigned by us. The sequences of the primers for Y-GATA-C4 were 5' -gtggaaccagcccaaatatc-3' and 5' -aatgctctcttggcttctcact-3'. Primers for Y-GATA-A10 were in accordance with White's (2). The PCR amplification conditions can be obtained at: http://www.legalmed.org/dna/y-c4a10.htm. The PCR reaction volume for each locus was 37.5 μL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (3). The diversity of haplotype, the discrimination power and the probability of exclusion for the two Y-STR loci were calculated according to Hou's method (4).


    Author Information:

    Tang, JP
    Institute of Forensic Medicine, Sichuan University, Chengdu,

    Wu, J
    Institute of Forensic Medicine, Sichuan University, Chengdu,

    Zhang, J
    Institute of Forensic Medicine, Sichuan University, Chengdu,

    Li, YB
    Institute of Forensic Medicine, Sichuan University, Chengdu,

    Hou, YP
    Institute of Forensic Medicine, Sichuan University, Chengdu,


    Stock #: JFS15236J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15236J

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    Author
    Title Haplotype Frequencies for Two New Y-STR Loci in Chinese Population
    Symposium , 0000-00-00
    Committee E30