Detection of Ketosis in Vitreous at Autopsy after Embalming

    Volume 47, Issue 1 (January 2002)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 2002

    Page Count: 3


    Volk, A
    University of Alabama at Birmingham, Birmingham, AL

    Robinson, CA

    Steinhauer, JR
    University of Alabama at Birmingham, Birmingham, AL

    Hardy, R
    University of Alabama at Birmingham, Birmingham, AL

    Konrad, R
    University of Alabama at Birmingham, Birmingham, AL

    Daly, T
    University of Alabama at Birmingham, Birmingham, AL

    (Received 29 December 2000; accepted 22 May 2001)

    Abstract

    Ketosis occurs in ketoacidosis or malnourishment. When either is suspected in relation to a death, it may be important to analyze for ketosis at autopsy. We encountered a case where starvation was suspected in a deceased nursing home resident, where the body had been embalmed prior to autopsy. Gas chromatography (GC) was unable to separate acetone from formaldehyde, a component of embalming fluid. The Acetest is a simple test that can detect acetone and acetoacetate in body fluids. We validated the Acetest with GC on vitreous. The Acetest and GC were consistent except at very low levels of acetone or acetoacetate. The sensitivity of the Acetest for acetoacetate in vitreous was 10 mg/dL, consistent with early starvation. Significant interference from embalming fluid did not occur. The Acetest was negative in the described case. The Acetest is a simple and useful test for the detection of ketosis in embalmed autopsies.


    Paper ID: JFS15231J

    DOI: 10.1520/JFS15231J

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    Author
    Title Detection of Ketosis in Vitreous at Autopsy after Embalming
    Symposium , 0000-00-00
    Committee E30