Volume 47, Issue 1 (January 2002)

    Typing Y Chromosome STR Haplotypes Using Redesigned Primers

    (Received 13 October 2000; accepted 3 April 2001)

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    A panel of Y-specific STR loci, including DYS19, DYS389, DYS390, DYS391, DYS392, and DYS393 was analyzed using horizontal nondenaturing polyacrylamide gel electrophoresis with a discontinuous buffer system (horizontal disk-PAGE). In order to obtain correct results for the larger DYS389 and DYS392 alleles, it was necessary to design new primers that bind closer to the repeat region and lead to a significant reduction of the amplified fragment size. Using the modified primer sets the horizontal disk PAGE results were consistent with a nondenaturing approach using fluorescent primers and a 377 automated sequencer. The modified procedure also amplifies the second repeat stretch at the duplicated DYS389 locus as a single fragment, which results in an immediate allele identification. The results indicate that horizontal disk-PAGE with silverstaining is a simple approach to type the recommended Y-specific STR markers.


    Author Information:

    Sun, D
    Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu,

    Wu, J
    Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu,

    Zhang, J
    Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu,

    Prinz, M
    OCME, New York, NY

    Zhang, S
    Sichuan University, Chengdu,

    Hou, Y
    Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu,

    Li, Y
    Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu,


    Stock #: JFS15229J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15229J

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    Author
    Title Typing Y Chromosome STR Haplotypes Using Redesigned Primers
    Symposium , 0000-00-00
    Committee E30