ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 2002
Page Count: 3

Typing Y Chromosome STR Haplotypes Using Redesigned Primers
Sun, D
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences),

Wu, J
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences),

Zhang, J
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences),

Prinz, M
OCME, NY

Zhang, S
Sichuan University,

Hou, Y
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences),

Li, Y
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences),

(Received 13 October 2000; accepted 3 April 2001)

Abstract

A panel of Y-specific STR loci, including DYS19, DYS389, DYS390, DYS391, DYS392, and DYS393 was analyzed using horizontal nondenaturing polyacrylamide gel electrophoresis with a discontinuous buffer system (horizontal disk-PAGE). In order to obtain correct results for the larger DYS389 and DYS392 alleles, it was necessary to design new primers that bind closer to the repeat region and lead to a significant reduction of the amplified fragment size. Using the modified primer sets the horizontal disk PAGE results were consistent with a nondenaturing approach using fluorescent primers and a 377 automated sequencer. The modified procedure also amplifies the second repeat stretch at the duplicated DYS389 locus as a single fragment, which results in an immediate allele identification. The results indicate that horizontal disk-PAGE with silverstaining is a simple approach to type the recommended Y-specific STR markers.

Keywords:
forensic science, chromosome Y, horizontal polyacrylamide gel electrophoresis, primers, short tandem repeats, haplotype

Paper ID: JFS15229J
DOI: 10.1520/JFS15229J
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