ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 2002
Page Count: 3

Typing of the Locus DYS19 from DNA Derived from Fingernail Clippings Using PCR Concert™ Rapid Purification System
Budowle, B
FBI, DC

Gangitano, DA
DNA Section,

Juvenal, GJ
CNEA-CONICET,

Garófalo, MG
CNEA-CONICET,

Padula, RA
DNA Section,

(Received 6 January 2001; accepted 22 May 2001)

Abstract

DNA extracted from the fingernails of female victims of a violent or aggressive act may assist in the identification of the male. Sometimes with the current autosomal STR loci, however, the victim's profile may mask the perpetrator's DNA profile or the perpetrator's DNA may be substantially lower in quantity than that of the victim's DNA. Thus, under these conditions, no characterization is possible. In this paper, an alternative DNA extraction procedure was employed, and the application of an STR locus residing on the Y chromosome DYS19 was typed to allow for genetic characterization of the perpetrator in such cases.

Keywords:
forensic science, DNA typing, fingernail clippings, polymerase chain reaction, short tandem repeats, Y chromosome, DYS19

Paper ID: JFS15220J
DOI: 10.1520/JFS15220J
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