ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

Recovery of DNA for Forensic Analysis from Lip Cosmetics
Turbett, GR
Forensic Biology Laboratory, Forensic Services, Western Australia

Webb, LG
Forensic Biology Laboratory, Forensic Services, Western Australia

Egan, SE
Forensic Biology Laboratory, Forensic Services, Western Australia

(Received 6 June 2000; accepted 7 February 2001)

Abstract

To obtain a reference DNA profile from a missing person, we analyzed a variety of personal effects, including two lip cosmetics, both of which gave full DNA profiles. Further investigations were undertaken to explore this previously unreported source of DNA. We have tested a range of brands and types of lip cosmetics. Our studies have revealed that lip cosmetics are an excellent source of DNA, with almost 80% of samples giving a result. However, artifacts are frequently observed in the DNA profiles when Chelex is used for the DNA extraction and additional DNA purification procedures are required to ensure that an accurate DNA profile is obtained.

Keywords:
forensic science, DNA extraction, polymerase chain reaction, DNA typing, Profiler Plus, human identification, short tandem repeat, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, amelogenin, cosmetic, lipstick

Paper ID: JFS15174J
DOI: 10.1520/JFS15174J
ASTM International is a member of CrossRef.