Volume 46, Issue 5 (September 2001)

    Detection of Cocaine and Its Metabolites in Breast Milk

    (Received 5 September 2000; accepted 11 January 2001)

    CODEN: JFSOAD

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    Abstract

    A method was developed for measuring cocaine and its metabolites, benzoylecgonine, ecgonine methyl ester, norcocaine, ecgonine ethyl ester, cocaethylene, and m-hydroxybenzoylecgonine, in breast milk by gas chromatography/mass spectrometry. Limits of detection for this method ranged from 2.5 to 10 ng/mL, and limits of quantitation ranged from 5 to 50 ng/mL. For each of the compounds measured by this method, linear response was demonstrated to 750 ng/mL. Breast milk was collected from 11 mothers who admitted to drug use during pregnancy and ten drug-free volunteers serving as control subjects. Cocaine was detected in six of the specimens obtained from drug-exposed subjects, and in none of the drug-free control subjects. In breast milk specimens where cocaine and one or more of its metabolites were detected, the concentration of parent compound was greater than any of the metabolites. The highest cocaine concentration found was over 12 μg/mL. Breast-fed infants of cocaine abusing mothers may be exposed to significant amounts of drug orally.


    Author Information:

    Goldberger, BA
    Immunology and Laboratory Medicine, Gainesville, FL

    Phillips, D
    Eli Lilly and Company, Indianapolis, IL

    Eyler, FD
    University of Florida College of Medicine, Gainesville, FL

    Tebbett, IR
    University of Florida College of Veterinary Medicine, Gainesville, FL

    Karlix, JL
    University of Florida College of Pharmacy, Gamesville, FL

    Wobie, K
    University of Florida College of Medicine, Gainesville, FL

    Behnke, M
    University of Florida College of Medicine, Gainesville, FL

    Conlon, M
    University of Florida, Gainesville, FL

    Winecker, RE
    Immunology and Laboratory Medicine, Gainesville, FL

    Bertholf, RL
    University of Florida Health Science Center, Jacksonville, FL


    Stock #: JFS15124J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15124J

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    Author
    Title Detection of Cocaine and Its Metabolites in Breast Milk
    Symposium , 0000-00-00
    Committee E30