ISSN: 0022-1198
CODEN: JFSCA
Page Count: 8

TWGDAM Validation of the AmpFℓSTR Profiler Plus and AmpF∓STR COfiler STR Multiplex Systems Using Capillary Electrophoresis
Buel, E
Vermont Forensic Laboratory, VT

Walkinshaw, MA
Vermont Forensic Laboratory, VT

Schwartz, MB
Vermont Forensic Laboratory, VT

LaFountain, MJ
Vermont Forensic Laboratory, VT

Svete, PA
Vermont Forensic Laboratory, VT

(Received 19 July 2000; accepted 3 January 2001)

Abstract

Prior to forensic implementation, a profiling system requires validation following the recommendations presented by the Technical Working Group on DNA Analysis Methods (TWG-DAM). In this work two such systems, AmpFℓSTR Profiler Plus and AmpFℓSTR COfiler have been validated according to the guidelines provided by TWGDAM. Profiler Plus and COfiler simultaneously amplify nine and six STR loci respectively; both also amplify a portion of the amelogenin gene. Performance of the two STR multiplex systems under conditions set forth by TWGDAM was robust and reproducible, indicating that these systems are suitable for use in forensic analysis. Additionally, specific sections of the TWGDAM validation guidelines are especially valuable in terms of familiarizing users with particular limitations of the systems prior to taking on casework.

Keywords:
forensic science, DNA, polymerase chain reaction, AmpFℓSTR Profiler Plus, AmpFℓSTR COfiler, validation, capillary electrophoresis

Paper ID: JFS15120J
DOI: 10.1520/JFS15120J
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