Deposition of 7-Aminoflunitrazepam and Flunitrazepam in Hair After a Single Dose of Rohypnol®

    Volume 46, Issue 5 (September 2001)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 9


    Negrusz, A
    College of Pharmacy, University of Illinois at Chicago, Chicago, IL

    Moore, CM
    U.S. Drug Testing Laboratories, Inc., Des Plaines, IL

    Hinkel, KB
    College of Pharmacy, University of Illinois at Chicago, Chicago, IL

    Strong, MJ
    College of Medicine, University of Illinois at Chicago, Chicago, IL

    Verma, M
    College of Medicine, University of Illinois at Chicago, Chicago, IL

    Janicak, PG
    College of Medicine, University of Illinois at Chicago, Chicago, IL

    Stockham, TL
    Ft. Lauderdale, FL

    (Received 17 August 2000; accepted 17 October 2000)

    Abstract

    In recent years, there has been a notable increase in the number of reports on drug-facilitated sexual assault. Benzodiazepines are the most common so-called “date-rape” drugs, with flunitrazepam (Rohypnol®) being one of the most frequently mentioned. The aim of this study was to determine whether flunitrazepam and its major metabolite 7-aminoflunitrazepam could be detected in hair collected from ten healthy volunteers after receiving a single 2 mg dose of Rohypnol® using solid phase extraction and NCI-GC-MS. Such data would be of great importance to law enforcement agencies trying to determine the best time interval for hair collection from a victim of drug-facilitated sexual assault in order to reveal drug use. Ten healthy volunteers (eight women and two men, 21 to 49 years old) participated in the study. The following hair samples were collected from each volunteer: one before flunitrazepam administration, and 1, 3, 5, 14, 21, and 28 days after. In five volunteers, 7-aminoflunitrazepam was detected 24 h after flunitrazepam administration and remained in hair throughout the entire 28-day study period (0.6–8.0 pg/mg). In two cases, 7-aminoflunitrazepam appeared in hair 21 days after drug intake (0.5–2.7 pg/mg), and in two subjects 14 days later (0.5–5.4 pg/mg). In one volunteer, 7-aminoflunitrazepam was detected on day 14 and 21 but concentrations were below the quantitation limit. Flunitrazepam was detected in some samples but all concentrations were below the quantitation limit (0.5–2.3 pg/mg).


    Paper ID: JFS15113J

    DOI: 10.1520/JFS15113J

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    Title Deposition of 7-Aminoflunitrazepam and Flunitrazepam in Hair After a Single Dose of Rohypnol®
    Symposium , 0000-00-00
    Committee E30