ISSN: 0022-1198
CODEN: JFSCA
Page Count: 2

Allele Frequencies for the PowerPlex™ 16 STR Loci in an Albanian Population Sample from Northern Italy
Robino, C
Dipartimento di Anatomia, Farmacologia e Medicina Legale, Lab. di Scienze Criminalistiche, Università degli Studi di Torino,

Torre, C
Dipartimento di Anatomia, Farmacologia e Medicina Legale, Lab. di Scienze Criminalistiche, Università degli Studi di Torino,

Gino, S
Dipartimento di Anatomia, Farmacologia e Medicina Legale, Lab. di Scienze Criminalistiche, Università degli Studi di Torino,

Abstract

In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint™ PowerPlex™ System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm

Keywords:
forensic science, DNA typing, population genetics, short tandem repeats, polymerase chain reaction, D3S 1358, TH01, D21S11, D18S51, PENTA E, D5S818, D13S317, D7S820, D16S539, CSF1PO, PENTA D, vWA, D8S1179, TPOX, FGA, Italy, Albanians

Paper ID: JFS15086J
DOI: 10.1520/JFS15086J
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