Detection of Benzodiazepines in Different Tissues, Including Bone, Using a Quantitative ELISA Assay

    Volume 46, Issue 4 (July 2001)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 3


    Melbye, FJ
    Forensic Science Section, University of Toronto, Toronto, Ontario

    Gorczynski, LY
    Forensic Science Section, University of Toronto, Toronto, Ontario

    (Received 3 March 2000; accepted 12 September 2000)

    Abstract

    Benzodiazepines were analyzed in different tissue samples, including bone, by ELISA. The sensitivity of detection for different benzodiazepines was consistent with the manufacturer's reports of the cross reactivities of the antibodies used, with the greatest sensitivity for midazolam and the least for diazepam; in addition the pharmacokinetics was consistent with the known duration of action of the different benzodiazepines, with midazolam cleared rapidly, and diazepam slowly. Following intramuscular injection of 300 μg of midazolam at 16 h intervals for ten days, the drug was detectable in bone tissue samples obtained from skeletonized remains buried in soil at room temperature for three weeks.


    Paper ID: JFS15069J

    DOI: 10.1520/JFS15069J

    ASTM International
    is a member of CrossRef.

    Author
    Title Detection of Benzodiazepines in Different Tissues, Including Bone, Using a Quantitative ELISA Assay
    Symposium , 0000-00-00
    Committee E30