Validation of the AmpFℓSTR™ Profiler Plus PCR Amplification Kit for Use in Forensic Casework

    Volume 46, Issue 3 (May 2001)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 5


    Riech, AK
    Illinois State Police, Research and Development Laboratory, Springfield, IL

    Llewellyn, BE
    Illinois State Police, Research and Development Laboratory, Springfield, IL

    Gandor, DW
    Illinois State Police, Research and Development Laboratory, Springfield, IL

    Parker, D
    Illinois State Police, Research and Development Laboratory, Springfield, IL

    Fish, PA
    Illinois State Police, Research and Development Laboratory, Springfield, IL

    Carter, RR
    Illinois State Police, Research and Development Laboratory, Springfield, IL

    Thibault, SM
    Illinois State Police, Research and Development Laboratory, Springfield, IL

    Marcacci, TL
    Illinois State Police, Research and Development Laboratory, Springfield, IL

    Frank, WE
    Illinois State Police, Research and Development Laboratory, Springfield, IL

    (Received 27 April 1999; accepted 13 June 2000)

    Abstract

    According to TWGDAM guideline 4.5 (1), prior to implementing a new DNA analysis procedure or an existing DNA analysis procedure developed by another laboratory, the forensic laboratory must first demonstrate reliability of the procedure inhouse. Seven phases were designed to validate the use of the AmpFℓSTR Profiler Plus PCR Amplification Kit, as well as the PE Applied Biosystems 310 Genetic Analyzer. This report summarizes the results obtained for each of the seven phases of the validation study which included the following evaluations: polymer, reproducibility, sensitivity, stutter, precision, mixtures and nonprobative casework.


    Paper ID: JFS15017J

    DOI: 10.1520/JFS15017J

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    Author
    Title Validation of the AmpFℓSTR™ Profiler Plus PCR Amplification Kit for Use in Forensic Casework
    Symposium , 0000-00-00
    Committee E30