Concordance Study on Population Database Samples Using the PowerPlex™ 16 Kit and AmpFℓSTR® Profiler Plus™ Kit and AmpFℓSTR® COfiler™ Kit

    Volume 46, Issue 3 (May 2001)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 5


    Budowle, B
    Senior scientist, FSRTC Laboratory Division, FBI Academy, Quantico, VA

    Sprecher, CJ
    Senior scientist, Promega Corporation, Madison, WI

    (Received 16 June 2000; accepted 21 July 2000)

    Abstract

    Over 500 population database samples comprising African Americans, Bahamians, and Southwestern Hispanics were typed using the PowerPlex™ 16 and the Profiler Plus™ COfiler™ kits. There was only one sample in which a typing difference was observed. An FGA heterozygote profile was observed using the PowerPlex™ 16 primers, and a single allele FGA profile was observed using Profiler Plus primers. Thus, the extant data suggest that the primers used in the PowerPlex™ 16, Profiler Plus™, and COfiler™ kits are reliable for typing reference samples destined for use in CODIS. In addition, African American, Bahamian, and Southwestern Hispanic databases have been established for the STR loci Penta D and Penta E. Both loci are highly polymorphic. The application of the product rule is valid for estimating the rarity of a multiple loci profile consisting of these two and the 13 core STR loci.


    Paper ID: JFS15016J

    DOI: 10.1520/JFS15016J

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    Title Concordance Study on Population Database Samples Using the PowerPlex™ 16 Kit and AmpFℓSTR® Profiler Plus™ Kit and AmpFℓSTR® COfiler™ Kit
    Symposium , 0000-00-00
    Committee E30