ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

Concordance Study on Population Database Samples Using the PowerPlex™ 16 Kit and AmpFℓSTR® Profiler Plus™ Kit and AmpFℓSTR® COfiler™ Kit
Budowle, B
Senior scientist, FSRTC Laboratory Division, FBI Academy, VA

Sprecher, CJ
Senior scientist, Promega Corporation, WI

(Received 16 June 2000; accepted 21 July 2000)

Abstract

Over 500 population database samples comprising African Americans, Bahamians, and Southwestern Hispanics were typed using the PowerPlex™ 16 and the Profiler Plus™ COfiler™ kits. There was only one sample in which a typing difference was observed. An FGA heterozygote profile was observed using the PowerPlex™ 16 primers, and a single allele FGA profile was observed using Profiler Plus primers. Thus, the extant data suggest that the primers used in the PowerPlex™ 16, Profiler Plus™, and COfiler™ kits are reliable for typing reference samples destined for use in CODIS. In addition, African American, Bahamian, and Southwestern Hispanic databases have been established for the STR loci Penta D and Penta E. Both loci are highly polymorphic. The application of the product rule is valid for estimating the rarity of a multiple loci profile consisting of these two and the 13 core STR loci.

Keywords:
forensic science, AmpFℓSTR® Profiler Plus™ Kit, AmpFℓSTR® COfiler™ Kit, PowerPlex™ 16 kit, GenePrint® PowerPlex™ 2.1 kit, CODIS, concordance, primers, STR, stochastic effects, allele drop-out, Penta D, Penta E, Hardy-Weinberg expectations

Paper ID: JFS15016J
DOI: 10.1520/JFS15016J
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