Volume 46, Issue 3 (May 2001)

    Determination of Drug Levels in Two Species of Necrophagous Coleoptera Reared on Substrates Containing Morphine

    (Received 1 February 2000; accepted 22 June 2000)

    CODEN: JFSOAD

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    Abstract

    Two species of necrophagous Coleoptera: Dermestes frischi (Dermestidae) and Thanatophilus sinuatus (Silphidae), were reared on substrates containing different amounts of morphine. Colonies of D. frischi were reared on rabbit carcasses which had been given 10, 20, and 40 mg/h of morphine hydrochloride via ear artery perfusion over a 3 h period prior to death. A fourth rabbit served as a control. T. sinuatus was reared on minced beef spiked with morphine hydrochloride to give concentrations of 1000, 2500, 5000, and 10 000 ng/g and one control colony. These dosages were calculated to create tissue concentrations of morphine similar to those encountered in human deaths due to morphine overdose. Larvae, pupae, and adults (except for T. sinuatus) were analyzed for morphine content. All developmental stages of D. frischi were positive for morphine and concentrations correlated with cadaveric tissue concentrations during larval stages and to a lesser extent in the adult stage. For T. sinuatus, the best correlations were found in 2nd and 3rd instar larvae. This study demonstrates the potential for use of necrophagous Coleoptera, as well as Diptera larvae, as alternate specimens for toxicological analyses.


    Author Information:

    Bourel, B

    Gosset, D
    Institut de Médecine Légale et Sociale, Faculté de Médecine, Place Théo Varlet, Lille,

    Tournel, G
    Institut de Médecine Légale et Sociale, Faculté de Médecine, Place Théo Varlet, Lille,

    Lee Goff, ML
    University of Hawaii at Manoa, Honolulu, HI

    Hédouin, V
    Institut de Médecine Légale et Sociale, Faculté de Médecine, Place Théo Varlet, Lille,


    Stock #: JFS15010J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15010J

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    Title Determination of Drug Levels in Two Species of Necrophagous Coleoptera Reared on Substrates Containing Morphine
    Symposium , 0000-00-00
    Committee E30