Volume 46, Issue 2 (March 2001)

    The Application of Minisatellite Variant Repeat Mapping by PCR (MVR-PCR) in a Paternity Case Showing False Exclusion Due to STR Mutation

    (Received 7 March 2000; accepted 10 May 2000)

    CODEN: JFSOAD

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    Abstract

    A boy and a girl with their mother brought a paternity suit against an alleged but deceased father. We tested six conventional genetic markers, the AmpliType PM+DQA1 and twelve STR loci the children and mother together with the alleged paternal grandparents. We also DNA typed the bloodstain found later in the alleged father's medical record. Only the result at D3S1358 in a nineplex STR system excluded the alleged father from parentage of the boy, whereas all markers were inclusive for the girl. Accordingly, we performed sequence analysis at D3S1358 to confirm the presence of a paternal exclusion or mutation. The sequence analysis indicated that the boy's allele 17 could have originated from either of the alleged father's allele 16 or 18 by a single-step mutation associated with slippage mutation in STR loci. We carried out minisatellite variant repeat mapping by PCR (MVR-PCR) at loci D1S8 (MS32) and D7S21 (MS31A) and mapped allele haplotypes of all individuals except the deceased alleged father. The MVR-PCR analysis showed that the boy has no inconsistency with the relationship between the alleged grandparents, and was very effective at increasing the paternity index (PI) value. We conclude that there is biological relationship between not only the girl but also the boy and the alleged father.


    Author Information:

    Jeffreys, AJ
    Professor, University of Leicester, Leicester,

    Tamaki, K
    Professor, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo,

    Mizutani, M
    Associate professor, research student, postgraduate student, assistant professor and professor, Postgraduate School of Medicine, Nagoya University, Nagoya,

    Uchihi, R
    Associate professor, research student, postgraduate student, assistant professor and professor, Postgraduate School of Medicine, Nagoya University, Nagoya,

    Yamamoto, T
    Associate professor, research student, postgraduate student, assistant professor and professor, Postgraduate School of Medicine, Nagoya University, Nagoya,

    Yoshimoto, T
    Associate professor, research student, postgraduate student, assistant professor and professor, Postgraduate School of Medicine, Nagoya University, Nagoya,

    Huang, X-L
    Postdoctral fellow, Tokai University School of Medicine, Bohseidai, Isehara,

    Katsumata, Y
    Associate professor, research student, postgraduate student, assistant professor and professor, Postgraduate School of Medicine, Nagoya University, Nagoya,


    Stock #: JFS14976J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14976J

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    Author
    Title The Application of Minisatellite Variant Repeat Mapping by PCR (MVR-PCR) in a Paternity Case Showing False Exclusion Due to STR Mutation
    Symposium , 0000-00-00
    Committee E30