Journal Published Online: 01 March 2001
Volume 46, Issue 2

Cytological Detection of Spermatozoa: Comparison of Three Staining Methods

CODEN: JFSCAS

Abstract

Sperm detection can be an important factor in confirming sexual assault in cases of rape. This paper compares three of the most commonly used staining methods cited in the scientific literature: Christmas tree, hematoxylin-eosin, and alkaline fuchsin. The population studied was composed of 174 consenting women seen at the Male Infertility Center in Toulouse, France. The date of their last sexual intercourse was accurately known. Alkaline fuchsin did not seem effective in detecting spermatozoa in vaginal samples. Compared with hematoxylin-eosin, Christmas tree stain appeared to be the most useful test in the first 72 h. Two external factors were associated with decreased detection of spermatozoa: time since intercourse and sperm volume.

Author Information

Allery, J-P
Rangueil University Hospital, Toulouse Cedex 4, France
Telmon, N
Rangueil University Hospital, Toulouse Cedex 4, France
Mieusset, R
Male Infertility Center, La Grave University Hospital, Toulouse Cedex, France
Blanc, A
Rangueil University Hospital, Toulouse Cedex 4, France
Rougé, D
Rangueil University Hospital, Toulouse Cedex 4, France
Pages: 3
Price: $25.00
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Stock #: JFS14970J
ISSN: 0022-1198
DOI: 10.1520/JFS14970J