ISSN: 0022-1198
CODEN: JFSCA
Page Count: 3

Identifying Sex Chromosome Abnormalities in Forensic DNA Testing Using Amelogenin and Sex Chromosome Short Tandem Repeats
Young, DR
Forensic DNA analyst, Texas Department of Public Safety Crime Laboratory, Texas

Honda, K
Research assistant, associate professor, professor, Osaka University Graduate School of Medicine,

Matoba, R
Research assistant, associate professor, professor, Osaka University Graduate School of Medicine,

Tun, Z
Research assistant, associate professor, professor, Osaka University Graduate School of Medicine,

(Received 17 December 1999; accepted 11 April 2000)

Abstract

Forensic DNA laboratories worldwide have begun using multiplexed STR systems to decrease analysis time and increase sample throughput. The loci used in these systems are basically “nonsense” regions of human DNA. However, due to the chromosome on which some of these loci are located, various genetic abnormalities can sometimes be detected. This paper will show one such abnormality—Klinefelter's Syndrome—and the process used to show the possibility of this defect in two undiagnosed males using peak height ratios at the Amelogenin locus, and X-Y STRs.

Keywords:
forensic science, DNA typing, short tandem repeat, amelogenin, X chromosome, Y chromosome, polymerase chain reaction, Klinefelter's Syndrome

Paper ID: JFS14969J
DOI: 10.1520/JFS14969J
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