Volume 45, Issue 6 (November 2000)

    Detection and Correction of a Migration Anomaly on a 310 Genetic Analyzer

    (Received 26 August 1999; accepted 25 January 2000)

    CODEN: JFSOAD

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    Abstract

    During STR analysis on the 310 Genetic Analyzer, retarded migration of GS500ROX size standards and alleles in some samples was observed. The contribution of reagents, capillary and performance optimized polymer POP 4 to the observed anomaly was experimentally eliminated. Variation in electrophoresis temperature between 55°C and 65°C did not alter the rate of migration of GX500ROX size standard and sample alleles. An eroded connector for the cathode mounted on the heat plate assembly caused the abnormal migration. Hence, it is important to verify the mobility of all fragments in the size standard for each sample to avoid any erroneous allele calls by the automated data analysis software.


    Author Information:

    Shewale, JG
    ReliaGene Technologies, Inc., New Orleans, LA

    Sinha, SK
    ReliaGene Technologies, Inc., New Orleans, LA

    Richey, SL
    ReliaGene Technologies, Inc., New Orleans, LA


    Stock #: JFS14893J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14893J

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    Author
    Title Detection and Correction of a Migration Anomaly on a 310 Genetic Analyzer
    Symposium , 0000-00-00
    Committee E30