Northeast Italy Population Data Using Multiplex PCR (HUMCD4, HUMTH01, HUMTPOX, and HUMCSF1P0) Loci

    Volume 45, Issue 6 (November 2000)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 3


    De Leo, D
    Postgraduate student, associate professor and director of Institute of Legal Medicine, and chancellor of University of Verona, Istituto di Medicina Legale, Lab. di Emogenetica Forense, Università degli Studi di Verona, Verona,

    Marigo, M
    Postgraduate student, associate professor and director of Institute of Legal Medicine, and chancellor of University of Verona, Istituto di Medicina Legale, Lab. di Emogenetica Forense, Università degli Studi di Verona, Verona,

    Turrina, S
    Postgraduate student, associate professor and director of Institute of Legal Medicine, and chancellor of University of Verona, Istituto di Medicina Legale, Lab. di Emogenetica Forense, Università degli Studi di Verona, Verona,

    (Received 17 May 1999; accepted 20 December 1999)

    Abstract

    Allele and genotype frequencies for four short tandem repeat (STR) loci (HUMCD4, HUMTH01, HUMTPOX, and HUMCSF1P0) were determined in 100 unrelated individuals from Veneto (Northeast Italy). After a multiplex polymerase chain reaction (PCR)-amplification, semi-automatic DNA profiling was performed using an A.L.F. express DNA Sequencer. Conditions were optimized for the PCR co-amplification of these four STR loci and the quadruplex PCR was performed on various forensic DNA samples such as whole blood, blood-stains, teeth, and saliva from Caucasians living in the Northeast Italy. The distribution of the genotype frequencies showed no significant deviation from Hardy-Weinberg expectations in the sampled population. The combined Power of Discrimination (PD) of the quadruplex was 0.9999.


    Paper ID: JFS14889J

    DOI: 10.1520/JFS14889J

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    Author
    Title Northeast Italy Population Data Using Multiplex PCR (HUMCD4, HUMTH01, HUMTPOX, and HUMCSF1P0) Loci
    Symposium , 0000-00-00
    Committee E30