Volume 45, Issue 5 (September 2000)

    Elimination of 7-Aminoflunitrazepam and Flunitrazepam in Urine After a Single Dose of Rohypnol®

    (Received 22 February 2000; accepted 7 April 2000)

    CODEN: JFSOAD

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    Abstract

    The hypnotic benzodiazepine flunitrazepam (Rohypnol®) has been identified as the drug of choice for the purposes of “drugging” unsuspecting victims and raping them while they are under the influence of this substance. The objective of this paper was to study elimination of flunitrazepam and 7-aminoflunitrazepam in urine collected from ten healthy volunteers who received a single 2 mg oral dose of Rohypnol®, to determine how long after drug administration 7-aminoflunitrazepam can be detected. A highly sensitive NCI-GC-MS method for the simultaneous quantitation of flunitrazepam (LOQ 100 pg/mL) and 7-aminoflunitrazepam (LOQ 10 pg/mL) in urine was developed. All samples were screened for benzodiazepines using optimized micro-plate enzyme immunoassay. The highest concentrations of 7-aminoflunitrazepam (70–518 ng/mL) and flunitrazepam (0.7–2.8 ng/mL) in urine were observed 6 h after drug administration in nine subjects and after 24 h in one subject. In six subjects 7-aminoflunitrazepam was detected up to 14 days after flunitrazepam administration, in one subject up to 21 days and in three subjects up to 28 days. In urine samples collected from six volunteers, flunitrazepam was detected three days after Rohypnol® intake, in three subjects 24 h, and in one subject 5 days later. Benzodiazepine micro-plate enzyme immunoassay kit allowed the detection of flunitrazepam and metabolities 5 to 21 days after drug administration.


    Author Information:

    Negrusz, A
    College of Pharmacy, University of Illinois at Chicago, Chicago, IL

    Moore, CM
    U.S. Drug Testing Laboratories, Inc., Des Plaines, IL

    Kern, JL
    College of Pharmacy, University of Illinois at Chicago, Chicago, IL

    Poiser, KR
    College of Pharmacy, University of Illinois at Chicago, Chicago, IL

    Levy, NA
    College of Medicine, University of Illinois at Chicago, Chicago, IL

    Le, NLT
    U.S. Drug Testing Laboratories, Inc., Des Plaines, IL

    Janicak, PG
    College of Medicine, University of Illinois at Chicago, Chicago, IL

    Palaparthy, R
    College of Pharmacy, University of Illinois at Chicago, Chicago, IL

    Stockham, TL
    Ft. Lauderdale, FL


    Stock #: JFS14827J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14827J

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    Author
    Title Elimination of 7-Aminoflunitrazepam and Flunitrazepam in Urine After a Single Dose of Rohypnol®
    Symposium , 0000-00-00
    Committee E30