ISSN: 0022-1198
CODEN: JFSCA
Page Count: 3

Sequence Variation of New Alleles at the Short Tandem Repeat D19S253 Locus
Vieira-Silva, C
Forensic geneticists, director of Forensic Biology Laboratory, director of Institute of Legal Medicine and research supervisor, Institute of Legal Medicine,

Geada, H

Pinto-Ribeiro, I

Viriato, L
Forensic geneticists, director of Forensic Biology Laboratory, director of Institute of Legal Medicine and research supervisor, Institute of Legal Medicine,

Brito, RM
Forensic geneticists, director of Forensic Biology Laboratory, director of Institute of Legal Medicine and research supervisor, Institute of Legal Medicine,

Espinheira, R
Forensic geneticists, director of Forensic Biology Laboratory, director of Institute of Legal Medicine and research supervisor, Institute of Legal Medicine,

Ribeiro, T
Forensic geneticists, director of Forensic Biology Laboratory, director of Institute of Legal Medicine and research supervisor, Institute of Legal Medicine,

(Received 7 June 1999; accepted 31 August 1999)

Abstract

This paper reports the sequences of two new alleles identified in a population database study on the short tandem repeat D19S253 locus. A Portuguese Caucasian population and a Portuguese African population were studied. Forty-four selected alleles were sequenced and 11 different alleles were found. All the sequenced alleles shown to possess a simple tetranucleotide GATA repeat region structure. The two new alleles, alleles 6 and 16, follow the simple repeat pattern. During paternity investigation casework, 1028 meiosis were analyzed and five isolated genetic incompatibilities detected. In one case, a non-detectable allele with the used set of primers could be the explanation. In the other four cases, single-step mutations could be considered. The mutation rate obtained for this locus was 3.89 × 10⁻³.

Keywords:
forensic science, DNA typing, short tandem repeat, paternity, D19S253, sequencing, mutation, new alleles

Paper ID: JFS14803J
DOI: 10.1520/JFS14803J
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