Volume 45, Issue 4 (July 2000)

    The Analysis of Three Short Tandem Repeat (STR) Loci in the Slovene Population by Multiplex PCR

    (Received 20 April 1999; accepted 7 September 1999)

    CODEN: JFSOAD

      Format Pages Price  
    PDF Version 3 $25   ADD TO CART


    Abstract

    Allele frequencies for three tetrameric short tandem repeat (STR) loci D3S1358, HUMVWA, and HUMFGA were determined in a Slovene Caucasian population sample. DNA samples from a total of 221 Slovenes were amplified by multiplex PCR using the commercial kit AmpFISTR Blue (Perkin-Elmer). Separation and detection of the amplified STR fragments were carried out using a 377 automated genetic analyzer (Applied Biosystem Division/Perkin Elmer). Seven alleles at the D3S1358 locus, 8 alleles at the HUMVWA31A locus, and 13 alleles at the HUMFGA locus were observed. A deviation from Hardy-Weinberg equilibrium was observed, only at the HUMVWA31A locus (p = 0.045, exact test). The departure at this locus was not significant after Bonferroni correction. There were no detectable departures between pairwise comparisons of the loci. The combined power of discrimination for all three loci is 0.9998, and the power of exclusion is 0.9526. The observed allele frequencies for the loci D3S1358, IIUMVWA31A, and HUMFGA are similar to those in European and U.S. Caucasian populations.


    Author Information:

    Budowle, B
    Senior scientist, Biology, Laboratory Division, FBI Academy, Quantico, VA

    Drobnič, K
    Forensic Science Laboratory, Ministry of the Interior, Ljubljana,


    Stock #: JFS14792J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14792J

    ASTM International
    is a member of CrossRef.

    Author
    Title The Analysis of Three Short Tandem Repeat (STR) Loci in the Slovene Population by Multiplex PCR
    Symposium , 0000-00-00
    Committee E30