You are being redirected because this document is part of your ASTM Compass® subscription.
    This document is part of your ASTM Compass® subscription.

    Volume 45, Issue 4 (July 2000)

    Gelatinase A in Human Dentin as a New Biochemical Marker for Age Estimation

    (Received 17 March 1999; accepted 27 September 1999)

    CODEN: JFSOAD

      Format Pages Price  
    PDF 5 $25   ADD TO CART

    Cite this document

    X Add email address send
    X
      .RIS For RefWorks, EndNote, ProCite, Reference Manager, Zoteo, and many others.   .DOCX For Microsoft Word



    Abstract

    Dentin proteins from 24 individual permanent molars from patients aged 15 to 73 years were sequentially extracted, with guanidinium chloride (G1-extract) and then with EDTA; after demineralization, the material was again extracted with guanidinium chloride (G2-extract). Extracts were analyzed by SDS-PAGE electrophoresis and the gels were processed for zymography to detect gelatinolytic activities. The patterns of gelatinase A distribution differed in the different dentin protein fractions, and the changes varied with age. Significant differences were detected in gelatinase A in G2-extracts between individual younger than 20 years old and the rest of the sample (xexp2 ,429; 1 d.f.; p < 0.001). The incidence of true and false positives and negatives, and sensitivity and specificity for the presence of gelatinase A in dentin extracts, were calculated. Determination of gelatinase A in human dentin may be a useful marker to estimate age, especially when other morphological methods are of limited usefulness.


    Author Information:

    Valenzuela, A
    Assistant professor and associate professor, Faculty of Medicine University of Granada, Granada,

    Overall, CM
    Faculty of Dentistry, University of British Columbia, Vancouver,

    Martín-de las Heras, S
    Assistant professor and associate professor, Faculty of Medicine University of Granada, Granada,


    Stock #: JFS14774J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14774J

    ASTM International
    is a member of CrossRef.

    Author
    Title Gelatinase A in Human Dentin as a New Biochemical Marker for Age Estimation
    Symposium , 0000-00-00
    Committee E30