ABTS: A Safe Alternative to DAB for the Enhancement of Blood Fingerprints

    Volume 45, Issue 4 (July 2000)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 10


    Caldwell, JP
    Student and senior lecturers, University of Waikato, Hamilton, New Zealand

    Kim, ND
    Student and senior lecturers, University of Waikato, Hamilton, New Zealand

    Henderson, W
    Student and senior lecturers, University of Waikato, Hamilton, New Zealand

    (Received 18 June 1999; accepted 21 September 1999)

    Abstract

    The use of 2,2′-azino-di-[3-ethylbenzthiazolinesulfonate(6)] diammonium salt (ABTS) for the enhancement of fingerprints in blood has been investigated. Optimal pH conditions and H2O2 concentrations have been determined using UV/Vis spectroscopy. ABTS is an effective and safe noncarcinogenic (though more expensive) alternative to the presently used 3,3′-diaminobenzidine (DAB) for the development of blood fingerprints, especially on porous surfaces. The bright green color of the oxidized ABTS is an advantage on certain colored surfaces where the dark brown color of DAB does not stand out well from the background. Development with ABTS does not interfere with subsequent DAB treatment, making ABTS a “nothing-to-lose” reagent. ABTS can be used after ninhydrin treatment, but the reverse is not the case.


    Paper ID: JFS14771J

    DOI: 10.1520/JFS14771J

    ASTM International
    is a member of CrossRef.

    Author
    Title ABTS: A Safe Alternative to DAB for the Enhancement of Blood Fingerprints
    Symposium , 0000-00-00
    Committee E30