ISSN: 0022-1198
CODEN: JFSCA
Page Count: 4

Population Studies and Validation of Paternity Determinations by Six Microsatellite Loci
Sigurdsson, A
University Hospital of Iceland,

Geirsson, G
University Hospital of Iceland,

Ingvarsson, S
University Hospital of Iceland,

Finnsdottir, V
University Hospital of Iceland,

(Received 12 May 1999; accepted 16 July 1999)

Abstract

A single locus system of 6 microsatellite markers was evaluated for paternity testing. A nonradioactive method based on peroxidase labeling of a DNA probe was used to estimate the allele frequency of markers D1S216, D3S1217, D7S480, D9S157, D13S153, and D16S422 by genotyping 1134–1698 chromosomes. The number of detected alleles were 22, 15, 23, 10, 16, and 19, respectively, and the allele frequency varied from 0.001 to 0.317. The genotype of 87 families, consisting of mother, father, and child was determined. The probability that a random individual will give a positive paternity was evaluated. We conclude that the markers can be reliably typed and give sufficient and reliable information for paternity testing.

Keywords:
forensic science, paternity, short tandem repeat, dinucleotide, polymerase chain reaction, forensic DNA typing, D1S216, D3S1217, D7S480, D9S157, D13S153, D16S422

Paper ID: JFS14751J
DOI: 10.1520/JFS14751J
ASTM International is a member of CrossRef.