Usefulness of a Toothbrush as a Source of Evidential DNA for Typing

    Volume 45, Issue 3 (May 2000)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 3


    Nozawa, H
    Associate Professor, Hamamatsu University School of Medicine, Hamamatsu,

    Ohtaki, H
    Postgraduate Student, Research Students, Associate Professor, and Professor, Postgraduate School of Medicine, Nagoya University, Nagoya,

    Sato, K
    Research Student and Professor, Showa University School of Medicine, Tokyo,

    Tamaki, K
    Lecturer, University of Leicester, Adrian Building, University Road, Leicester,

    Tanaka, M
    Postgraduate Student, Research Students, Associate Professor, and Professor, Postgraduate School of Medicine, Nagoya University, Nagoya,

    Yamamoto, T
    Postgraduate Student, Research Students, Associate Professor, and Professor, Postgraduate School of Medicine, Nagoya University, Nagoya,

    Yoshimoto, T
    Postgraduate Student, Research Students, Associate Professor, and Professor, Postgraduate School of Medicine, Nagoya University, Nagoya,

    Kato, Y
    Research Student and Professor, Showa University School of Medicine, Tokyo,

    Katsumata, Y
    Postgraduate Student, Research Students, Associate Professor, and Professor, Postgraduate School of Medicine, Nagoya University, Nagoya,

    (Received 26 January 1999; accepted 2 August 1999)

    Abstract

    We investigated the usefulness of a toothbrush as a source of DNA for an unidentified cadaver. Ten toothbrushes were obtained from ten individuals along with their peripheral blood. We recovered from 10 to 430 ng of DNA from all but one of the toothbrushes. All ten toothbrushes, including the one containing no detectable DNA by fluorometry, were typed correctly at all of the loci tested, including nine STRs. Three toothbrushes obtained in two actual deaths also identified two victims and one suspect. Therefore, toothbrushes seem to be useful as a source of evidential DNA for personal identification.


    Paper ID: JFS14746J

    DOI: 10.1520/JFS14746J

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    Title Usefulness of a Toothbrush as a Source of Evidential DNA for Typing
    Symposium , 0000-00-00
    Committee E30