ISSN: 0022-1198
CODEN: JFSCA
Page Count: 2

Interferences with Semen Detection by an Immunoassay for a Seminal Vesicle-Specific Antigen
Hortin, GL
Medical technician/student and acting chief for clinical chemistry, National Institutes of Health, MD

Chen, JT
Medical technician/student and acting chief for clinical chemistry, National Institutes of Health, MD

(Received 11 January 1999; accepted 5 April 1999)

Abstract

A commercial enzyme-linked immunosorbent assay (ELISA), the SEMA® assay, for a seminal vesicle-specific antigen (SVSA) provides highly sensitive detection of semen. Here we show marked interference of proteins such as albumin, serum proteins, or mucin with the assay. This would substantially decrease the sensitivity for detecting semen mixed with other biological fluids such as blood or vaginal secretions.

Keywords:
forensic science, semen, identification, seminal vesicles, immunoassay seminal vesicle specific antigen

Paper ID: JFS14670J
DOI: 10.1520/JFS14670J
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