ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

Determination of Amphetamine by HPLC After Acetylation
Veress, T
Institute for Forensic Sciences,

(Received 22 October 1998; accepted 24 May 1999)

Abstract

An analytical procedure has been developed for the HPLC determination of amphetamine by off-line pre-column derivatization. The proposed procedure consists of sample preparation by acetylation of amphetamine with acetic anhydride and a subsequent reversed-phase HPLC separation on an octadecyl silica stationary phase with salt-free mobile phase (tetrahydrofuran, acetonitrile, 0.1% triethylamine in water, 15:15:70 v/v) applying UV-detection. The applicability of the elaborated procedure is demonstrated with results obtained by analysis of real samples seized in the Hungarian black market.

Keywords:
forensic science, amphetamine, acetylation, derivatization, determination, high-performance liquid chromatography, substance abuse

Paper ID: JFS14652J
DOI: 10.1520/JFS14652J
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