Volume 45, Issue 1 (January 2000)

    Determination of Amphetamine by HPLC After Acetylation

    (Received 22 October 1998; accepted 24 May 1999)


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    An analytical procedure has been developed for the HPLC determination of amphetamine by off-line pre-column derivatization. The proposed procedure consists of sample preparation by acetylation of amphetamine with acetic anhydride and a subsequent reversed-phase HPLC separation on an octadecyl silica stationary phase with salt-free mobile phase (tetrahydrofuran, acetonitrile, 0.1% triethylamine in water, 15:15:70 v/v) applying UV-detection. The applicability of the elaborated procedure is demonstrated with results obtained by analysis of real samples seized in the Hungarian black market.

    Author Information:

    Veress, T
    Institute for Forensic Sciences, Budapest,

    Stock #: JFS14652J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14652J

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    Title Determination of Amphetamine by HPLC After Acetylation
    Symposium , 0000-00-00
    Committee E30