Volume 44, Issue 6 (November 1999)

    Frequencies of D8S384 Alleles and Genotypes in European, African-American, Chinese, and Japanese Populations

    (Received 17 September 1998; accepted 26 February 1999)

    CODEN: JFSOAD

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    Abstract

    D8S384 is a tetranucleotide tandem repeat locus. In order to evaluate the forensic validation of D8S384, the genotype distributions and allele frequencies in ten populations from three main ethnic groups were investigated, including Germans, Slovakians, African Americans, Japanese, and Chinese (Jilin, Guangzhou, Nanning, Hailaer, Dali, and Chengdu). A total of 1011 unrelated individuals, 41 pedigrees, 30 disputed paternity trios and three personal identification cases were analyzed for D8S384 by Amp-FLP technique. Many kinds of tissues, body fluids, secreta and stains have been tested. The alleles were determined by comparison with a human allele ladder. The results showed that D8S384 typing was both precise and reliable. There were eight alleles in these populations. The genotype distributions conformed to Hardy-Weinberg equilibrium predictions. No mutation events were observed. With a maximum likelihood method, the mutation rate was indirectly estimated as 2.14 × 10−5. The heterozygosity was 0.704 ± 0.014 at D8S384 locus. All these results suggest that D8S384 locus is a useful marker for forensic identification and paternity analysis.


    Author Information:

    Chen, GD
    Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu, Sichuan

    Meng, HY
    Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu, Sichuan

    Walter, H
    University of Bremen, Bremen,

    Wu, J
    Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu, Sichuan

    Prinz, M
    Department of Forensic Biology, OCME, New York, NY

    Li, YB
    Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu, Sichuan

    Hou, YP
    Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu, Sichuan


    Stock #: JFS14600J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14600J

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    Title Frequencies of D8S384 Alleles and Genotypes in European, African-American, Chinese, and Japanese Populations
    Symposium , 0000-00-00
    Committee E30