The Potential Contribution of MVR-PCR to Paternity Probabilities in a Case Lacking a Mother

    Volume 44, Issue 4 (July 1999)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 5


    Jeffreys, AJ
    Professor, University of Leicester, Leicester,

    Tamaki, K
    Associate professor, postgraduate students, associate professor, student, assistant professor and professor, Nagoya University School of Medicine, Nagoya,

    Mizutani, M
    Associate professor, postgraduate students, associate professor, student, assistant professor and professor, Nagoya University School of Medicine, Nagoya,

    Katsumata, R
    Associate professor, postgraduate students, associate professor, student, assistant professor and professor, Nagoya University School of Medicine, Nagoya,

    Uchihi, R
    Associate professor, postgraduate students, associate professor, student, assistant professor and professor, Nagoya University School of Medicine, Nagoya,

    Yamamoto, T
    Associate professor, postgraduate students, associate professor, student, assistant professor and professor, Nagoya University School of Medicine, Nagoya,

    Huang, X-L
    Associate professor, postgraduate students, associate professor, student, assistant professor and professor, Nagoya University School of Medicine, Nagoya,

    Katsumata, Y
    Associate professor, postgraduate students, associate professor, student, assistant professor and professor, Nagoya University School of Medicine, Nagoya,

    (Received 7 August 1998; accepted 2 November 1998)

    Abstract

    Minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) was applied to a paternity case lacking a mother to evaluate the paternity probability. After three flanking polymorphic sites at each of MS31A and MS32 loci were investigated from the child and alleged father, allele-specific MVR-PCR was performed using genomic DNA. It was confirmed that one allele in the child was identical to that in the alleged father at both loci. Mapped allele codes were compared with allele structures established from population surveys. No perfect matches were found although some motifs were shared with other Japanese alleles. The paternity index and probability of paternity exclusion at these two MVR loci were then estimated, establishing the power of MVR-PCR even in paternity cases lacking a mother.


    Paper ID: JFS14569J

    DOI: 10.1520/JFS14569J

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    Title The Potential Contribution of MVR-PCR to Paternity Probabilities in a Case Lacking a Mother
    Symposium , 0000-00-00
    Committee E30