Journal Published Online: 01 July 1999
Volume 44, Issue 4

The Potential Contribution of MVR-PCR to Paternity Probabilities in a Case Lacking a Mother

CODEN: JFSCAS

Abstract

Minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) was applied to a paternity case lacking a mother to evaluate the paternity probability. After three flanking polymorphic sites at each of MS31A and MS32 loci were investigated from the child and alleged father, allele-specific MVR-PCR was performed using genomic DNA. It was confirmed that one allele in the child was identical to that in the alleged father at both loci. Mapped allele codes were compared with allele structures established from population surveys. No perfect matches were found although some motifs were shared with other Japanese alleles. The paternity index and probability of paternity exclusion at these two MVR loci were then estimated, establishing the power of MVR-PCR even in paternity cases lacking a mother.

Author Information

Tamaki, K
Nagoya University School of Medicine, Nagoya, Japan
Huang, X-L
Nagoya University School of Medicine, Nagoya, Japan
Mizutani, M
Nagoya University School of Medicine, Nagoya, Japan
Yamamoto, T
Nagoya University School of Medicine, Nagoya, Japan
Katsumata, R
Nagoya University School of Medicine, Nagoya, Japan
Uchihi, R
Nagoya University School of Medicine, Nagoya, Japan
Katsumata, Y
Nagoya University School of Medicine, Nagoya, Japan
Jeffreys, AJ
University of Leicester, Leicester, U.K.
Pages: 5
Price: $25.00
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Stock #: JFS14569J
ISSN: 0022-1198
DOI: 10.1520/JFS14569J