Volume 44, Issue 4 (July 1999)

    Electrospray and MALDI Mass Spectrometry in the Identification of Spermicides in Criminal Investigations

    (Received 2 September 1998; accepted 17 November 1998)

    CODEN: JFSOAD

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    Abstract

    Electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry have been used to examine evidence in a sexual assault investigation. Because condoms are being used increasingly by sexual assailants and some condom brands include the spermicide nonoxynol-9 (nonylphenoxy polyethoxyethanol) in the lubricant formulation, the recovery, and identification of nonoxynol-9 from evidence items may assist in proving corpus delicti. A method was developed for the recovery of nonoxynol-9 from internal vaginal swabs and for its identification by reverse phase liquid chromatography/electrospray ionization mass spectrometry (LC ESI-MS), nanoelectrospray ionization (nanoESI) mass spectrometry, and high resolution MALDI Fourier transform mass spectrometry (MALDI-FTMS). The method was tested on extracts from precoitus, immediate postcoitus, and four-hours postcoitus vaginal swabs provided by a volunteer whose partner does not normally use condoms, but for this trial used a condom having a water-soluble gel-type lubricant that includes 5% nonoxynol-9 in its formulation. Subsequently, LC ESI-MS was used to identify traces of nonoxynol-9 from the internal vaginal swab of a victim of a sexual assault.


    Author Information:

    Siuzdak, G
    The Scripps Research Institute, Beckman Center for Chemical Sciences, La Jolla, CA

    Blackledge, RD
    United States Naval Criminal Investigative Service, Regional Forensic Laboratory, San Diego, California

    Hollenbeck, TPE
    The Scripps Research Institute, Beckman Center for Chemical Sciences, La Jolla, CA


    Stock #: JFS14553J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14553J

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    Author
    Title Electrospray and MALDI Mass Spectrometry in the Identification of Spermicides in Criminal Investigations
    Symposium , 0000-00-00
    Committee E30